Comparative effects of neonatal DES on external genitalia development in adult male mice

Many of the effects of DES, including the induction of hypospadias, are due to impaired growth and tissue fusion events during development

Highlights:

Elsevier logo image
This 2014 study suggests that many of the effects of DES, including the induction of hypospadias, are due to impaired growth and tissue fusion events during development.
  • Prenatal DES treatment of C57BL/6 and CD-1 mice elicited a broad spectrum of penile and preputial malformations that were consistently more severed in C57BL/6 mice having enhanced estrogen sensitivity.
  • Adverse effects of DES correlated with the expression of estrogen receptors within the affected tissues.
  • The developmental basis of several adult DES-induced malformations was presented.

Abstract:

Neonatally DES-induced penile and preputial hypospadias are suggested to be due to impaired growth and tissue fusion events during development.

The effect of neonatal exposure to diethylstilbestrol (DES), a potent synthetic estrogen, was examined to evaluate whether the CD-1 (estrogen insensitive, outbred) and C57 (estrogen sensitive, inbred) mouse strains differ in their response to estrogen disruption of male ExG differentiation.

CD-1 and C57BL/6 litters were injected with sesame oil or DES (200ng/g/5μl in sesame oil vehicle) every other day from birth to day 10. Animals were sacrificed at the following time points: birth, 5, 10 and 60 days postnatal.

Neonatally DES-treated mice from both strains had many ExG abnormalities that included the following:

  • severe truncation of the prepuce and glans penis,
  • an abnormal urethral meatus,
  • ventral tethering of the penis,
  • reduced os penis length and glans width,
  • impaired differentiation of cartilage,
  • absence of urethral flaps,
  • impaired differentiation of erectile bodies.

Adverse effects of DES correlated with the expression of estrogen receptors within the affected tissues. While the effects of DES were similar in the more estrogen-sensitive C57BL/6 mice versus the less estrogen-sensitive CD-1 mice, the severity of DES effects was consistently greater in C57BL/6 mice.

We suggest that many of the effects of DES, including the induction of hypospadias, are due to impaired growth and tissue fusion events during development.

Sources

  • Comparative effects of neonatal diethylstilbestrol on external genitalia development in adult males of two mouse strains with differential estrogen sensitivity,
    ScienceDirect, stories/4607851, 18 Oct 2014.
  • Full text: Elsevier, article/pii/S0301468114000541, DOI: 10.1016/j.diff.2014.09.004.
More DES DiEthylStilbestrol Resources

Author: DES Daughter

Activist, blogger and social media addict committed to shedding light on a global health scandal and dedicated to raise DES awareness.

Have your say! Share your views

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s

This site uses Akismet to reduce spam. Learn how your comment data is processed.