57 Different Pesticides found in Poisoned Honeybees

Honeybees are being poisoned – new test reveals how

Honeybee-Poisoning image
A new method that can detect a large range of pesticides in bees could help scientists work out what is causing the global decline in honey bees. A study using the method found up to 57 different pesticides and digested pesticide compounds in poisoned honey bees.


57 Different Pesticides Found in Poisoned Honeybees, elsevier, February 10, 2016.

A method for the determination of 200 pesticides and pesticide metabolites in honeybee samples has been developed and validated.

Almost 98% of compounds included in this method are approved to use within European Union, as active substances of plant protection products or veterinary medicinal products used by beekeepers to control mites Varroa destructor in hives.

Many significant metabolites, like metabolites of imidacloprid, thiacloprid, fipronil, methiocarb and amitraz, are also possible to detect.

Honeybees are being poisoned – new test reveals how, elsevier, 10 March 2016.

The sample preparation was based on the buffered QuEChERS method. Samples of bees were extracted with acetonitrile containing 1% acetic acid and then subjected to clean-up by dispersive solid phase extraction (dSPE) using a new Z-Sep+ sorbent and PSA. The majority of pesticides, including neonicotionoids and their metabolites, were analyzed by liquid chromatography tandem mass spectrometry (LC–MS/MS) but some of pesticides, especially pyrethroid insecticides, were analyzed by gas chromatography tandem mass spectrometry (GC–MS/MS). The procedure was validated according to the Guidance document SANCO/12571/2013 at four concentration levels: 1, 5, 10 and 100 ng/g bees and verified in the international proficiency test.

Multi-residue method for the determination of pesticides and pesticide metabolites in honeybees by liquid and gas chromatography coupled with tandem mass spectrometry—Honeybee poisoning incidents, Journal of Chromatography A on sciencedirect, S0021967316300012, February 2016.

The analysis of bee samples spiked at the limit of quantification (LOQ) showed about 98% mean recovery value (trueness) and 97% of analytes showed recovery in the required range of 70–120% and RSDr (precision) below 20%. Linearity and matrix effects were also established. The LOQs of pesticides were in the range of 1–100 ng/g.

The developed method allows determination of insecticides at concentrations of 10 ng/g or less, except abamectin and tebufenozide. LOQ values are lower than the median lethal doses LD50 for bees.

The method was used to investigate more than 70 honeybee poisoning incidents. Data about detected pesticides and their metabolites are included.

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