Long term surveillance of DES exposed women is a must
2015 Study Abstract
Clear cell adenocarcinoma of the cervix is a rare tumor of the lower genital tract. It has been described in young women with a history of intra uterine exposure to diethylstilbestrol. This tumor is characterized by a greater tendency for late recurrences. In this article, we report the case of one exposed-patient who developed recurrence as liver metastases, 24 years after the initial treatment. This case demonstrates the need and the importance for continued follow-up in individuals prenatally exposed to diethylstilbestrol.
2015 Study Conclusions
To summarize, this case represents the longest reported disease-free interval till recurrence and the first description of metastatic liver disease of DES related clear cell adenocarcinoma of the cervix. It reemphasizes the necessity of long term surveillance of DES exposed women and confirms previous reports recommending the importance of frequent follow-up examination not only of the pelvis but also of all distant potential sites of metastasis. It also shows that treatment with paclitaxel, carboplatin and bevacizumab can be an effective and safe therapeutic option for treating recurrence of this rare tumor.
Sources and more information
Very late recurrence of Diethylstilbestrol – related clear cell carcinoma of the cervix: case report,Ablavi Adani-Ifè, Emma Goldschmidt, Pasquale Innominato, Ayhan Ulusakarya, Hassan Errihani, Philippe Bertheau and Jean François Morère, Gynecologic Oncology Research and Practice 2015, $article.volume.volumeNumber:3 doi:10.1186/s40661-015-0010-5, gynoncrp.com/content/2/1/3, 17 July 2015.
A retrospective review was conducted at 3 major gynecologic cancer centers of all primary CCCC between 1982 and 2004.
CCCC was confirmed in 34 patients. Median age was 53 years. DES exposure was confirmed in 2 (6%) patients. A history of smoking was elicited in 22%, and of abnormal Pap smear 18% patients. Primary surgical resection was performed in all stage I or IIA patients (n=26); they displayed superior 3-year overall survival (OS) compared to advanced stage (n=8) patients (91% vs. 22%, p<0.001). Pelvic lymph node involvement was noted in 25%; all patients with positive para-aortic nodes (20% of patients sampled) had positive pelvic nodes. All node positive patients were treated with adjuvant radiation, but nevertheless displayed reduced progression free (31% vs 92%, p<0.001) and overall survival (80% vs. 100%, p=0.02). Adjuvant radiotherapy did not appear to impact OS in patients with negative lymph nodes.
This series provides insight into the management of early stage CCCC in the post-DES era. Although these patients may be at slightly higher risk of nodal spread, clear cell histology by itself does not appear to portend a worse prognosis than squamous cell carcinoma of the cervix in the absence of traditional risk factors. Our data suggest that patients with low risk early stage CCCC may be managed with radical surgery alone, without the need for adjuvant chemotherapy or radiation.
Sources and more information
Clear cell carcinoma of the cervix: A multi-institutional review in the post-DES era, M. Bijoy Thomas, Jason D. Wright, Aliza L. Leiser, Dennis S. Chi, David G. Mutch, Karl C. Podratz, and Sean C. Dowdya,, Gynecol Oncol. 10.1016/j.ygyno.2008.02.007, NCBI PMC3667392, 2008 Apr 3.
Developmental exposure to DES alters uterine gene expression that may be associated with uterine neoplasia later in life
Previously, we described a mouse model where the well-known reproductive carcinogen with estrogenic activity, diethylstilbestrol (DES), caused uterine adenocarcinoma following neonatal treatment. Tumor incidence was dose-dependent reaching >90% by 18 mo following neonatal treatment with 1000 μg/kg/d of DES. These tumors followed the initiation/promotion model of hormonal carcinogenesis with developmental exposure as initiator, and exposure to ovarian hormones at puberty as the promoter. To identify molecular pathways involved in DES-initiation events, uterine gene expression profiles were examined in prepubertal mice exposed to DES (1, 10, or 1000 μg/kg/d) on days 1–5 and compared to controls. Of more than 20 000 transcripts, approximately 3% were differentially expressed in at least one DES treatment group compared to controls; some transcripts demonstrated dose–responsiveness. Assessment of gene ontology annotation revealed alterations in genes associated with cell growth, differentiation, and adhesion. When expression profiles were compared to published studies of uteri from 5-d-old DES-treated mice, or adult mice treated with 17β estradiol, similarities were seen suggesting persistent differential expression of estrogen responsive genes following developmental diethylstilbestrol exposure. Moreover, several altered genes were identified in human uterine adenocarcinomas. Four altered genes [lactotransferrin (Ltf), transforming growth factor beta inducible (Tgfb1), cyclin D1 (Ccnd1), and secreted frizzled-related protein 4 (Sfrp4)], selected for real-time RT-PCR analysis, correlated well with the directionality of the microarray data. These data suggested altered gene expression profiles observed 2 wk after treatment ceased, were established at the time of developmental exposure and maybe related to the initiation events resulting in carcinogenesis.
NCBI, Dr Retha Newbold, PMCID: PMC2254327 25 Feb 2008 – Developmental exposure to diethylstilbestrol alters uterine gene expression that may be associated with uterine neoplasia later in life.
In utero DES exposure associated with severe clear-cell adenocarcinoma in DES Daughters
A variety of vaginal and cervical abnormalities have been encountered in the offspring of women who have taken stilbestrol or chemically related nonsteroidal estrogens during pregnancy.
Cervical erosion has been noted most often, but vaginal adenosis has been proven by biopsy in over 30 percent, and transverse vaginal and cervical ridges have been seen in approximately 10 percent of the exposed population. Although the use of these drugs had been widespread during the last two decades, the Registry of Clear-Cell Adenocarcinoma of the Genital Tract in Young Females has been able to collect only 170 cases of vaginal and cervical cancers of this type from all over the world.
It is important that cytologists and pathologists become familiar with the various non-enoplastic and neoplastic disorders related to these hormones in order that additional epidemiologic, clinical and pathological information be acquired without delay.
DES has terrible side-effects in DES Daughters, DES Sons, DES Grand-Children…
Intrauterine exposure to diethylstilbestrol (DES) has been associated with this subsequent rare development of clear cell adenocarcinoma of the vagina and cervix in exposed female progeny. These tumors have been seen thus far in patients between the ages of 7 and 29 years. In addition, nonmalignant epithelial and structural genital alterations have been frequently observed. The epithelial changes of adenosis, ectropion, metaplasia, and potential neoplasia are considered. Thus far, no increased risk of squamous cell neoplasia has been demonstrated in DES-exposed subjects. However, increased pregnancy wastage, including premature birth, does appear to be more common among DES-exposed women; this increase may be related to the structural uterine and cervical changes that have occurred. DES-exposed men have been demonstrated to have anatomic genital changes, but the effects of these changes, if any, on male infertility are not known. Masculinization of the female external genitalia, including phallic enlargement and labioscrotal fusion, has been reported following intrauterine exposure to certain progestational agents and androgens.
The majority of these mothers who said they did not take DES were indeed DES positive by written records…
The written obstetric records of maternal exposure to diethylstilbestrol (DES) were used as a criterion standard and compared with the DES exposure history recalled by mothers of women with vaginal, cervical, or indeterminable vaginal/exocervical clear cell adenocarcinoma.
Among cervical cases, the sensitivity of maternal recall was 50% (N = 2), and its specificity was 100%. Among vaginal and vaginal/exocervical cases, this sensitivity was 72%; specificity was 60%; and the majority of these mothers who said they did not take DES were DES positive by written records.
Thus investigators should avoid using maternal recall alone to measure DES exposure. Among subjects for whom written maternal obstetric records were available, 88% of vaginal cases and 46% of cervical cases were DES positive. The authors conclude that few cases of vaginal clear cell adenocarcinoma should occur in young women as the cohort of women exposed in utero to DES continues to age, whereas cases of cervical origin may continue to occur.
A total of 143 exposed women and 49 unexposed women in the DES Combined Cohort Follow-up Study were diagnosed with cancer as of 2001.
Compared with breast cancer rates in the general US population, there was no overall higher risk among DES exposed women. Comparing exposed with unexposed women within the study, there was about a 30% increase in cancer risk but this finding could be due to chance. As reported in a previous article (Palmer 2006), breast cancer risk was elevated but only among women over 40 years of age. Exposed women had a risk of CCA that was nearly 40% higher than the general population, however; the incidence of CCA decreased substantially after age 25 compared with women 20 to 24 years old. Excluding CCA and breast cancer, the higher risk of cancer among the DES exposed women was about 20%, a result that could be due to chance. DES was not associated with higher risks of either endometrial or ovarian cancer. These data suggest that the DES associated increase in CCA rates remains elevated through the reproductive years. There was no consistent evidence of a higher risk for cancers other than CCA, and breast in older women.
Given that the population is still young, continued follow-up is necessary to assess the overall cancer risk associated with prenatal DES exposure.
Cancer risk in women prenatally exposed to diethylstilbestrol,NCBI, PMID: 17390375, 2007 Jul 15;121(2):356-60.
Currently, over 400 cases of clear cell adenocarcinoma of the vagina and cervix occurring in females born after 1940 have been accessioned into the Registry for Research on Hormonal Transplacental Carcinogenesis. Cases have been identified throughout the United States as well as Australia, Canada, Mexico, and Europe. Intrauterine exposure to DES and similar nonsteroidal estrogens have been uncovered in about two-thirds of the cases with an available maternal history. A peak in the age incidence curve of the DES-related cases has been observed at about 19 years with the age range being 7-30 years. The five-year survival for 400 patients has been 80%. Numerous nonmalignant epithelial changes have been observed. It appears that there has not been an increase in the occurrence of premalignant or malignant squamous cell lesions among the DES exposed. Premature birth has been more common among DES-exposed women. For those in whom there is evidence of a midpregnancy loss or premature ripening of the cervix during pregnancy, a cerclage procedure has been effective in producing a desirable outcome.
The developing fetus and neonate are uniquely sensitive to exogenous estrogen exposure
2006 Study Abstract
The synthetic estrogen diethylstilbestrol (DES) is a potent perinatal endocrine disruptor. In humans and experimental animals, exposure to DES during critical periods of reproductive tract differentiation permanently alters estrogen target tissues and results in long-term abnormalities such as uterine neoplasia that are not manifested until later in life. Using the developmentally exposed DES mouse, multiple mechanisms have been identified that play a role in its carcinogenic and toxic effects. Analysis of the DES murine uterus reveals altered gene expression pathways that include an estrogen-regulated component. Thus, perinatal DES exposure, especially at low doses, offers the opportunity to study effects caused by weaker environmental estrogens and provides an example of the emerging scientific field termed the developmental origin of adult disease. As a model endocrine disruptor, it is of particular interest that even low doses of DES increase uterine tumor incidence. Additional studies have verified that DES is not unique; when other environmental estrogens are tested at equal estrogenic doses, developmental exposure results in increased incidence of uterine neoplasia similar to that caused by DES. Interestingly, our data suggest that this increased susceptibility for tumors is passed on from the maternal lineage to subsequent generations of male and female descendants; the mechanisms involved in these transgenerational events include genetic and epigenetic events. Together, our data point out the unique sensitivity of the developing organism to endocrine-disrupting chemicals, the occurrence of long-term effects after developmental exposure, and the possibility for adverse effects to be transmitted to subsequent generations.
OVER THE LAST decade, mounting evidence from wildlife, epidemiological, laboratory animal, and in vitro studies suggests that numerous environmental and dietary chemicals can interfere with an organism’s complex endocrine signaling mechanisms and result in adverse consequences, especially alterations in reproductive tract tissues and function (1, 2, 3, 4, 5, 6). Because reduced fertility and reproductive problems are well acknowledged consequences of estrogen exposure as demonstrated by the estrogen mimics o,p′-dichlorodiphenyltrichloroethane (DDT) (7), the phytoestrogens in clover (8), and the seminal observations of the adverse effects of diethylstilbestrol (DES) on human health (9), initial concern focused on chemicals with estrogenic activity; however, it has become increasingly evident that this is not the only endocrine mode of action that merits attention. Interest has broadened to include chemicals that mimic or interfere with the normal actions of all endocrine hormones including estrogens, androgens, progestins, and thyroid, hypothalamic, and pituitary hormones; these chemicals are now collectively referred to as endocrine disruptors. With over 80,000 chemicals in commercial use in the United States alone today, only a few have been tested for endocrine-disruptor activity. It is generally assumed that most of these chemicals are not likely to pose a significant health risk at the levels of environmental exposures that exist; however, in reality, the full extent of the health consequences of these chemicals is simply unknown. Furthermore, we are just beginning to understand how chemicals act as endocrine disruptors and to appreciate the complexities of endocrine signaling mechanisms.
Sensitivity of the Developing Organism and Developmental Basis of Adult Disease:
Although adult exposure to endocrine-disrupting chemicals is important, the focus on the fetus and/or neonate is of primary concern because developing organisms are extremely sensitive to perturbation by chemicals with hormone-like activity. For example, an adverse effect may be most pronounced in the developing organism and occur at concentrations of the chemical that are far below levels that would be considered harmful in the adult. The exquisite sensitivity of the developing fetus and neonate has been described by Howard Bern in a chapter titled The Fragile Fetus (10) and is suggested to be due to numerous factors including undeveloped DNA repair mechanisms, an immature immune system, lack of detoxifying enzymes, primitive liver metabolism, lack of the development of the blood/brain barrier, and an increased metabolic rate.
Unique problems may be encountered when studying chemical exposures of the fetus and neonate undergoing critical developmental windows of differentiation. 1) Extrapolation of risks may be difficult because effects may not follow a monotonic dose-response relationship typically seen in toxicity studies; for example, higher concentrations of chemicals may show no effect, whereas lower concentrations do exhibit an effect. 2) The test chemical may have an entirely different effect in the embryo, fetus, or perinatal organism compared with effects seen in adults. 3) Effects may be manifested in offspring but not in their exposed parent. 4) Timing of exposure in the developing organism is critical in determining the final outcome in the adult organism. 5) Although critical exposure occurs during embryonic/fetal/neonatal development, manifestation of the effects may not be seen until much later in life (the developmental basis of adult disease). Developmental exposure can thus lead to a number of adverse effects in adults, which may include tumors in endocrine target tissues and adverse reproductive effects in males and females.
The scientific hypothesis that adult health and disease have an etiology arising in fetal or early neonatal development is not unique to the field of endocrine disruption. In the late 1980s, reports gained prominence by suggesting that the fetal environment, as reflected by low birth size and poor nutrition, was related to increased risk of noncommunicable diseases later in adult life; associations with coronary heart disease quickly extended to include type 2 diabetes, osteoporosis, and metabolic dysfunction (11). These findings led to the development of the developmental origins of health and disease paradigm in which a substantial research effort focused on the perinatal influences on chronic disease (12, 13, 14). Perinatal effects are no longer viewed in terms of just teratogenic changes or acute birth injury such as thalidomide-induced limb malformations, but whether changes induced in early development (preimplantation through prepubertal stages) may lead to life-long anomalies. Certainly unique problems exist in studying chemical exposures during development and their relationship to adult disease, but despite these difficulties, research findings continue to support the idea that environmental chemicals, in particular those with estrogenic activity, can have endocrine-disrupting effects that result in long-term health consequences.
As an example, the profound effects of estrogens on the developing reproductive tract have been demonstrated by prenatal exposure to DES (for review, see Refs. 15, 16, 17). Although DES effects were well recognized and firmly documented long before the proposed developmental origins of health and disease paradigm, DES clearly demonstrates that chemical exposure, in addition to nutrition and other perinatal factors, can significantly alter the developing organism and cause long-term effects in the adult.
DES as a Model Estrogenic Endocrine Disruptor:
For almost 30 yr, clinicians prescribed DES to women with high-risk pregnancies to prevent miscarriages and other complications of pregnancy. In 1971, a clinical report associated DES with a rare form of reproductive tract cancer termed vaginal adenocarcinoma, which was detected in a small number (<0.1%) of adolescent daughters of women who had taken the drug while pregnant (9). Subsequently, DES was also linked to more frequent benign reproductive tract problems in an estimated 95% of the DES-exposed daughters; reproductive organ dysfunction, abnormal pregnancies, reduced fertility, and disorders of the immune system were reported. Similarly, DES-exposed male offspring demonstrated structural, functional, and cellular abnormalities after prenatal exposure including hypospadias, microphallus, retained testes, inflammation, and decreased fertility (for review, see Ref. 17). DES became the first example of an in utero estrogenic toxicant in humans; it was shown to cross the placenta and induce a direct effect on the developing fetus. Based on the medical catastrophe it caused, DES can be viewed as the original endocrine-disrupting chemical. DES is no longer used clinically to prevent miscarriage, but a major concern remains that, as DES-exposed women age and reach the time at which the incidence of reproductive organ cancers normally increase, they will show a much higher incidence of cancer than unexposed individuals. Furthermore, the possibility of second-generation effects has been reported (18, 19, 20, 21), which puts still another generation at risk for developing problems associated with DES treatment of their grandmothers. Unfortunately, the DES episode continues to have serious health consequences and serves as a reminder of the toxicities that can be caused by hormonally active chemicals.
To study the mechanisms involved in DES-induced teratogenesis and carcinogenesis, experimental animal models were developed to study the adverse effects of developmental exposure to environmental estrogens on reproductive tract development and differentiation; these models continue to be used to study effects of other endocrine-disrupting chemicals. The high doses that were used in many of the early animal studies were similar to doses administered to pregnant women and thus have clinical relevance; lower doses are informative when using DES as a model estrogenic endocrine disruptor. The prenatal DES-exposed mouse model has been particularly successful in replicating and predicting abnormalities reported in DES-exposed humans (22, 23, 24, 25, 26, 27). A comparison of DES abnormalities (Table 1⇓) in humans and mice demonstrates the usefulness of DES-exposed experimental animal models.
Although vaginal adenocarcinoma was the original lesion of clinical interest because of its rare occurrence in young DES-exposed women, we have focused much of our research on uterine adenocarcinoma because it provides the potential to study a lesion that increases with age and occurs at a high incidence. Realizing that reproductive tract differentiation continues into neonatal life for both humans and mice, outbred CD-1 mice were treated neonatally with DES (2 μg/pup·d) on d 1–5; a high incidence of uterine cancer (90–95%) was seen in mice at 18–24 months of age (28). These tumors rarely metastasized, but in aged animals (24 months of age or older), the lesions were observed to spread to para-aortic lymph nodes or directly extended to contiguous organs (28). Investigations with other species neonatally treated with DES including rats (29) and hamsters (30) also reported a high incidence of uterine tumors. Because other experimental rodent models have duplicated uterine tumors seen in the CD-1 mouse, this lesion may be predictive of the carcinogenic potential of environmental estrogens in women as they age. It is significant that these mouse tumors progressed through the same morphological and biological continuum of hyperplasia to atypical hyperplasia to neoplasia similar to that seen in women and that the tumors were histologically similar to the human pathologies (28).
To establish a dose-response curve for uterine adenocarcinoma useful for endocrine disruptor studies, neonatal mice were treated with varying doses of DES ranging from 0.0002–2 μg/pup·d. Few studies have covered these low doses (31, 32, 34). The results showed that DES caused tumors even at a dose of 0.0002 μg/pup·d (Table 2⇓). Unlike other estrogen-responsive end points published by our lab and others that showed an inverted U dose-response relationship (33), uterine cancer followed a linear dose-dependent response with increasing incidence of tumors after increasing dose.
Interestingly, tumor incidence could be predicted based on estrogenic potency seen during neonatal life. Figure 1A⇓ shows neonatal estrogenicity in response to varying doses of DES and its relationship to subsequent carcinogenicity (Fig. 1B⇓) observed in aged mice. Both graphs show a dose-dependent linear increase, unlike some other end points that have shown nonmonotonic responses. Furthermore, DES induced estrogen-regulated genes like uterine lactoferrin (LF) on neonatal d 5, and the induction followed a similar linear response as shown by Western blotting techniques (Fig. 2⇓); thus, LF may be useful to predict future carcinogenic effects. Whether LF is directly associated with carcinogenicity or is simply a marker remains to be determined, but it is a subject of study in our laboratory.
To determine whether DES was unique or whether other environmental estrogens could cause uterine lesions, neonatal mice were similarly treated on d 1–5 with 17β-estradiol, tamoxifen, hexestrol, tetrafluorodiethylstilbestrol, ethinyl estradiol, 2-hydroxyestradiol, 4-hydroxyestradiol, genistein, nonylphenol, bisphenol A, or methoxychlor. Most of the studies were conducted using 2 μg/pup·d based on previous studies using DES; however, the weaker environmental estrogens (genistein, nonylphenol, bisphenol A, and methoxychlor) were tested at a higher dose (200 μg/pup·d). All compounds except methoxychlor caused uterine lesions in aged mice; results are summarized in Table 3⇓. Methoxychlor (pure, not technical grade) was the only compound tested that did not cause uterine lesions. Because reports in the literature suggest that a metabolite of methoxychlor is estrogenic, not methoxychlor itself (35), and because the neonatal liver is not fully functional during the time of treatment (36), the most likely explanation for lack of tumors is that the compound was not metabolized to an active estrogen form. All other compounds were estrogenic, and all were associated with uterine tumors after developmental exposure.
Uterine carcinomas have not been observed in untreated control CD-1 mice at corresponding ages, at various stages of the estrous cycle, or after similar adult short-term exposure to estrogens. This suggests that the developmental stage of uterine differentiation and the time of estrogen exposure as well as the estrogenic potency are all important factors in the development of uterine lesions.
Numerous studies have demonstrated that developmental exposure to DES interferes with normal differentiation of the Müllerian duct and regression of the Wolffian duct. Although the mechanisms are not completely understood, a molecular component in the malformation of the tissues and perhaps in the cellular changes may be responsible. Developmental studies have reported that HOX genes are involved in the structural differentiation of the reproductive tract (37) and that prenatal DES delays the expression of these genes (38). Thus, this molecular misprogramming is apparently responsible for the structural alterations observed in the DES reproductive tract (39). Additional investigations with Wnt genes also suggest DES is working through multiple gene pathways to cause structural changes (40, 41, 42).
We have also described permanent abnormal gene imprinting, which may be involved in tumor induction and other cellular alterations in the reproductive tract; neonatal exposure to DES caused demethylation of the estrogen-responsive gene LF in the mouse uterus (43). Studies to determine altered methylation patterns in other estrogen-responsive genes continue.
Similar to tumors described in mice, the neonatal estrogen-exposed hamster developed uterine carcinoma at a high frequency after developmental exposure to DES (30). Molecular studies with the hamster concluded that imbalances in the estrogen-regulated uterine expression of c-jun, c-fos, c-myc, bax, bcl-2, and bcl-x protooncogenes probably played a role in the molecular mechanisms by which neonatal DES treatment ultimately induced epithelial neoplasia in the rodent uterus (44). Microarray studies with the murine uterus in our laboratory revealed similar altered gene expression pathways that included an estrogen-regulated component (Grissom, S., W. N. Jefferson, E. Padilla-Banks, E. Lobenhofer, and R. R. Newbold, submitted for publication).
The role of estrogen receptor (ER) in the induction of abnormalities and tumors after developmental DES exposure has been studied using transgenic mice that overexpressed ERα (MT-mER). MT-mER mice were treated with DES during neonatal life and followed as they aged. It was hypothesized that because of abnormal overexpression of ERα, reproductive tract tissues of the MT-mER mice might be more susceptible to tumors after neonatal DES treatment. This was indeed the case because mice overexpressing ERα were at a higher risk of developing abnormalities including uterine carcinoma in response to neonatal DES compared with DES-treated wild-type mice. At 8 months, 73% of the DES-treated MT-mER mice compared with 46% of the DES-treated wild-type mice had uterine tumors. Furthermore, these lesions occurred at an earlier age compared with wild-type DES mice (45). These transgenic mouse studies suggested that ERα levels present in a tissue may be a determining factor in the development of estrogen-related tumors. Additional transgenic mouse models that expressed variant forms of ERα, and DES-treated ER knockout mice that lacked uterine tumors, also suggested that ERα played a role in the development of reproductive tract lesions (40). ERβ was not identified in the murine uterus (46, 47); thus, its role in uterine abnormalities is unclear and requires additional study.
The role of metabolism in DES-induced lesions has long been an area of investigation. Using the DES mouse model, catechol estrogens, in particular 4-hydroxyestradiol, were very effective at uterine tumor induction (36). Although both 2- and 4-hydroxyestradiol were carcinogenic, the latter induced a 9-fold higher tumor incidence compared with the parent hormone estradiol (36). In addition to hormone-related cell proliferation that may be associated with DNA damage, 4-hydroxyestradiol can be further oxidized to a quinone reactive intermediate. Metabolic redox cycling between this quinone and the hydroquinone (4-hydroxyestradiol) may then produce mutagenic free radicals. Thus, estrogenic compounds may induce tumors in target tissues by inducing DNA damage and genetic lesions and by stimulating proliferation of cells damaged by such processes (36). Together, these data suggested that estrogens may be operating through multiple mechanisms to induce tumors.
Because mechanistic studies provided support that estrogens caused both genetic and epigenetic alterations in developing target tissues, the possibility was raised that abnormalities seen after prenatal or neonatal DES treatment could be transmitted to subsequent generations. In fact, studies from our laboratory showed that prenatal or neonatal treatment with DES led to tumors in the female and male genital tract, and in addition, the susceptibility for tumors was transmitted to the descendants through the maternal germ cell lineage (18, 19); transmission via the DES-exposed male was not studied. Mice were treated with DES prenatally (2.5, 5, or 10 μg/kg·d) on d 9–16 of gestation, or neonatally (0.002 μg/pup·d) on d 1–5, which were the highest doses that did not drastically interfere with fertility later in life. When female mice (F1) reached sexual maturity, they were bred to control untreated males. Female and male offspring (DES lineage or F2) from these matings were aged to 17–24 months and examined for genital tract abnormalities. An increased incidence of proliferative lesions of the rete testis (an estrogen target tissue in the male) and tumors of the reproductive tract was observed in DES-lineage males (18). Furthermore, in DES-lineage females, an increased incidence of uterine adenocarcinoma was seen (19). The incidence was lower in DES descendants than in their parents; uterine tumor incidence in DES F1 at 18 months was 31% at the neonatal dose of 0.002 (Table 2⇑), whereas it was 11% in their DES descendants (19). These data suggest that alterations occurred in germ cells and were passed to subsequent generations. Interestingly, multigenerational effects of DES have been reported by other laboratories, and some of these report transmission through the paternal lineage (21, 48).
The mechanisms involved in these transgenerational events are unknown, but altered methylation patterns can be transmitted to subsequent generations. We have shown altered methylation patterns in several uterine genes that are permanently dysregulated after developmental DES treatment (43, 49). The estrogen-responsive proteins LF and c-fos were permanently up-regulated in the uterus after developmental exposure to DES, and the promoter region of these genes was shown to be hypomethylated (43, 49). Although the consequences of these types of alterations are unclear, studies suggested that methylation patterns can be passed to subsequent generations (50). A recent report supports this theory because prenatal exposure to vinclozolin or methoxychlor caused adverse effects on testis morphology and male fertility, and these effects were transmitted to subsequent generations (51). In addition, this report showed that these two chemicals caused epigenetic alterations in the DNA, specifically hyper- and hypomethylation, and that these alterations were also observed in subsequent generations (51) (also see Anway and Skinner article in this series, Ref. 52). Because the response of estrogen-regulated genes is set during development, altered hormone response may be transmitted to subsequent generations.
Transgenerational effects may also be associated with alterations in specific estrogen-responsive genes. For example, LF induction in prepubertal females that were exposed neonatally to DES showed that this gene continued to be overexpressed even after treatment was completed (Fig. 3A⇓). Furthermore, this gene was also overexpressed in uterine tissues from DES-lineage females, although these mice never received DES (Fig. 3B⇓). Other estrogen-responsive genes are being similarly studied in DES-lineage mice, and thus far, these data suggest involvement of an altered gene expression pathway that includes an estrogen-regulated component. Future study into transgenerational effects of other environmental chemicals and the mechanisms that govern these effects is a newly emerging research focus that deserves serious attention.
Summary and Recommendations:
Sufficient evidence has been accumulated through the years in experimental animals and humans to show that the developing fetus and neonate are uniquely sensitive to exogenous estrogen exposure. If exposure occurs during critical periods of differentiation, permanent adverse effects are well documented to result. Some of these effects, such as reproductive tract abnormalities and uterine tumors, may not be observed until much later in life, long after exposure occurs. Most importantly, evidence with experimental animals suggests that adverse effects may be transmitted to subsequent generations; however, more studies are needed to determine whether this transmission of tumor potential occurs in humans. An important cohort to follow that would answer many of the unresolved questions for humans is the grandchildren of DES-exposed women. Furthermore, additional studies in both experimental animals and humans are needed to identify and understand the mechanisms involved in the transmission of disease and to detect early markers of subsequent disease.
Although animal studies must be considered carefully before extrapolation to humans follows, the DES-exposed mouse model has provided some interesting comparisons to similarly exposed humans. The model has duplicated and predicted many of the lesions observed in DES-exposed women. Although DES is a potent estrogen, it continues to provide markers of the adverse effects of exposure to estrogenic and other endocrine-disrupting substances during development, whether these exposures come from naturally occurring chemicals, from synthetic or environmental contaminants, or from pharmaceutical agents. Ongoing mechanistic studies will help identify other potential reproductive toxicants and will help better access the risks of exposure to other endocrine-disrupting chemicals in the environment if chemical exposures occur during critical stages of development.